Effects of Sample Load on Resolution (QA-825)

A mixture of transferrin and ovalbumin was injected into IEC QA-825 (a column for ion exchange chromatography) to obtain the relation between sample load and resolution(Rs). With up to 2 mg of sample load, the Rs remained at approximately 3 with no decline. Rs was 2.5 when the sample load was 5 mg. Even when the sample load was increased to 10 mg, Rs was approximately 2 with satisfactory resolution.


Sample :
Transferrin and Ovalbumin

Column       : Shodex IEC QA-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Piperazine-HCl buffer (pH6.0)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 20 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.

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