A mixture of transferrin and ovalbumin was injected into IEC QA-825 (a column for ion exchange chromatography) to obtain the relation between sample load and resolution(Rs). With up to 2 mg of sample load, the Rs remained at approximately 3 with no decline. Rs was 2.5 when the sample load was 5 mg. Even when the sample load was increased to 10 mg, Rs was approximately 2 with satisfactory resolution.
Sample :
Transferrin and Ovalbumin
Sample :
Transferrin and Ovalbumin
Column : Shodex IEC QA-825 (8.0 mm I.D. x 75 mm) Eluent : (A); 20 mM Piperazine-HCl buffer (pH6.0) (B); (A) + 0.5 M NaCl Linear gradient: 0 min to 20 min, (A) to (B) Flow rate : 1.0 mL/min Detector : UV (280 nm) Column temp. : Room temp.
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Recovery of Proteins and Enzime Activity (QA-825)
- Recovery of Proteins and Enzyme Activity (DEAE-825)
- Recovery of Proteins and Enzyme Activity (SP-825)
- Recovery of Proteins and Enzyme Activity (CM-825)
- Effects of Sample Load on Peak Width (DEAE-825)
- Effects of Sample Load on Peak Width (SP-825)
- Effects of Sample Load on Resolution (CM-825)
- Effects of pH on Elution Pattern (DEAE-825)
- Effects of pH on Elution Pattern (SP-825)
- Standard Proteins (5) (QA-825)
- Standard Proteins (6) (ES-502N 7C)
- Standard Proteins (7) (CM-825, SP-825)
- Standard Proteins (8) (ES-502C 7C)
- Crude Albumin (Chicken Egg)
- Human Serum (4) (DEAE-825)
- Proteins in Human Serum (1) (QA-825)
- Proteins in Human Serum (2) (DEAE-825)
- Lipoxidase from Soybean (1) (QA-825, DEAE-825)
- Collagen and BSA
- β Lactoglobulin Subfractions
- Angiotensin Ⅰ, Ⅱ, Ⅲ
- Lysozyme Chloride
- Cytochrome P450 in Rat Liver Microsome
- Myosin Subfragments
- Bovine Liver Catalase
- Purified Kallikrein from Pig Pancreas
- Ribonuclease A from Equine Pancreas (CM-825)
- Lipoxidase (1) (SP-825)
- Papain
- Mercaptpalbumin and Nonmercaptoalbumin by Ion Exchange Chromatography