Myosin subfragments were analyzed using Asahipak ES-502C 7C ( a column for weak cation exchange chromatography ).


Sample : Myosin subfragment

Column       : Shodex Asahipak ES-502C 7C (7.5 mm I.D. x 100 mm)
Eluent       : (A); 5 mM Sodium phosphate buffer (pH7.0)
               (B); (A) + 0.1 M NaCl
               Linear gradient: 0 min to 25 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : 10 °C

Courtesy of Dr. Muno, Juntendo University