Lipoxidase from Soybean (1) (QA-825, DEAE-825)

Lipoxidase from soybean was analyzed using IEC QA-825 (a column for strong anion exchange chromatography) and IEC DEAE-825 (a column for weak anion exchange chromatography). The enzyme activity of this sample was measured by using hydrogen peroxide as a substrate. Enzyme activity was found at the arrowhead position.

Sample: Lipoxidase from soybean

QA-825
Column
：Shodex IEC QA-825 (8.0 mm I.D. x 75 mm)
Eluent
：(A); 20 mM Ethanolamine-HCl buffer (pH9.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 0 min to 60 min, (A) to (B)
Flow rate
：0.6  mL/min
Detector
：UV (280 nm)
Column temp.
：Room temp.

 

DEAE-825
Column
：Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm)
Eluent
：(A); 20 mM Tris-HCl buffer (pH8.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 0 min to 60 min, (A) to (B)
Flow rate
：1.0  mL/min
Detector
：UV (280 nm)
Column temp.
：Room temp.

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