Six kinds of protien standard were analyzed using IEC CM-825 (a column for weak cation exchange chromatography) and IEC SP-825 (a column for strong cation exchange chromatography).
Sample:
- 1.Myoglobin
- 2.Trypsinogen
- 3.Ribonuclease A
- 4.α Chymotrypsinogen A
- 5.Cytochrome c
- 6.Lysozyme
- Column
- ：Shodex IEC CM-825, SP-825 (8.0 mm I.D. x 75 mm eacn)
- Eluent
- ：(A); 20 mM Sodium phosphate buffer (pH7.0)
(B); (A) + 0.5 M NaCl
Linear gradient; 0 min to 60 min, (A) to (B) - Flow rate
- ：0.6 mL/min
- Detector
- ：UV (280 nm)
- Column temp.
- ：Room temp.
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Recovery of Proteins and Enzime Activity (QA-825)
- Recovery of Proteins and Enzyme Activity (DEAE-825)
- Recovery of Proteins and Enzyme Activity (SP-825)
- Recovery of Proteins and Enzyme Activity (CM-825)
- Effects of Sample Load on Resolution (QA-825)
- Effects of Sample Load on Peak Width (DEAE-825)
- Effects of Sample Load on Peak Width (SP-825)
- Effects of Sample Load on Resolution (CM-825)
- Effects of pH on Elution Pattern (DEAE-825)
- Effects of pH on Elution Pattern (SP-825)
- Standard Proteins (5) (QA-825)
- Standard Proteins (6) (ES-502N 7C)
- Standard Proteins (8) (ES-502C 7C)
- Crude Albumin (Chicken Egg)
- Human Serum (4) (DEAE-825)
- Proteins in Human Serum (1) (QA-825)
- Proteins in Human Serum (2) (DEAE-825)
- Lipoxidase from Soybean (1) (QA-825, DEAE-825)
- Collagen and BSA
- β Lactoglobulin Subfractions
- Angiotensin Ⅰ, Ⅱ, Ⅲ
- Lysozyme Chloride
- Cytochrome P450 in Rat Liver Microsome
- Myosin Subfragments
- Bovine Liver Catalase
- Purified Kallikrein from Pig Pancreas
- Ribonuclease A from Equine Pancreas (CM-825)
- Lipoxidase (1) (SP-825)
- Papain
- Mercaptpalbumin and Nonmercaptoalbumin by Ion Exchange Chromatography