Six kinds of protien standard were analyzed using IEC CM-825 ( a column for weak cation exchange chromatography ) and IEC SP-825 ( a column for strong cation exchange chromatography ).


Sample :
1. Myoglobin, 2. Trypsinogen, 3. Ribonuclease A, 4. α Chymotrypsinogen A, 5. Cytochrome c, 6. Lysozyme

Columns      : (Left) Shodex IEC CM-825 (8.0 mm I.D. x 75 mm)
               (Right) Shodex IEC SP-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Sodium phosphate buffer (pH7.0)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 60 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.