Recovery of proteins | |
---|---|
Protein | Recovery (%) |
Trypsinogen | 93 |
α-Chymotrypsinogen A | 99 |
α-Chymotrypsin | 106 |
Myoglobin | 96 |
Lysozyme | 96 |
Ribonuclease A | 99 |
Cytochrome c | 100 |
γ-Globulin | 100 |
Recovery of enzyme activity | |
---|---|
Enzyme | Recovery (%) |
Trypsin | 90 |
α-Chymotrypsin | 102 |
Column : Shodex IEC SP-825 (8.0 mm I.D. x 75 mm) Eluent : (A); 20 mM Sodium phosphate buffer (pH6.0) (B); (A) + 0.5 M NaCl Linear gradient: 0 min to 1 min, (A) to (B)
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