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Recovery of Proteins and Enzyme Activity (SP-825)

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The tables show good recovery of IEC SP-825 (a column for strong cation exchange chromatography) for proteins and enzyme activity.

Recovery of proteins
ProteinRecovery (%)
Trypsinogen 93
α-Chymotrypsinogen A 99
α-Chymotrypsin 106
Myoglobin 96
Lysozyme 96
Ribonuclease A 99
Cytochrome c 100
γ-Globulin 100
Recovery of enzyme activity
EnzymeRecovery (%)
Trypsin 90
α-Chymotrypsin 102

Sample load : 0.5 mg each

Column : Shodex IEC SP-825 (8.0 mm I.D. x 75 mm)
Eluent : (A); 20 mM Sodium phosphate buffer (pH6.0)
         (B); (A) + 0.5 M NaCl
         Linear gradient: 0 min to 1 min, (A) to (B)

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