When IEC SP-825 (a column for strong cation exchange chromatography) is used, the peak does not widen until the sample load of ribonuclease A reaches 1 mg. When the quantity is increased to 5 mg, the peak widths approximately 1.5 times.


Sample : Ribonuclease A

Column       : Shodex IEC SP-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Sodium phosphate buffer (pH7.0)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 20 min, (A) to (B)
Detector     : UV (280 nm)
Column temp. : Room temp.