When IEC DEAE-825 (a column for weak anion exchange chromatography) is used, the peak does not widen until the sample load of ovalbumin reaches 1 mg. When the quantity is increased to 4 mg, the peak approximately doubles its width.


Sample : Ovalbumin

Column       : Shodex IEC DEAE-825 (8.0 mm I.D. x 75 mm)
Eluent       : (A); 20 mM Piperazine-HCl buffer (pH6.0)
               (B); (A) + 0.5 M NaCl
               Linear gradient: 0 min to 20 min, (A) to (B)
Flow rate    : 1.0 mL/min
Detector     : UV (280 nm)
Column temp .: Room temp.