Effects of Temperature on Retention Time (SI-36 4D)

Please use this column with suppressor type ion chromatography system.

The relationship between column temperature and retention time of each anion in the anion analysis column IC SI-36 4D is shown below. When the temperature is increased, the elution time of sulfate ions decreased.At 40 ℃ or higher, the elution orders of sulfate ion and bromide ion are reversed. Considering the elution time of carbonate, we recommend a column temperature of 30 ℃ and the use of 25 mM potassium hydroxide aqueous solution as eluent.The relationship between column temperature and retention time of each anion in the anion analysis column SI-36 4D is shown below. The elution behavior with temperature depends on the kind of anion. The higher the temperature, the slower the elution of sulfate ions. At 40 ℃ or higher, the elution orders of sulfate ion and bromide ion are reversed. Considering the elution time of carbonate, column temperature of 30 ℃ is recommended, when using 25 mM potassium hydroxide aqueous solution as eluent.

Sample: 25 μL

  1. 1.F- 0.5 mg/L
  2. 2.Cl- 3 mg/L
  3. 3.NO2- 5 mg/L
  4. 4.SO42- 10 mg/L
  5. 5.Br- 10 mg/L
  6. 6.NO3- 10 mg/L
  7. 7.PO33- 20 mg/L
Column
Shodex SI-36 4D (4.0 mm I.D. x 150 mm)
Eluent
25 mM KOH aq.
(Eluent source : DionexTM EGC 500 KOH)
Flow rate
0.7 mL/min
Detector
Suppressed conductivity
Column temp.
30. 35, 40, 45, 50 ℃

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