Separation of Phosphate, Phosphtite and Hypophosphite (SI-90 4E)

Please use this column with suppressor type ion chromatography system.

This shows the analytical conditions to separate Phosphate(HPO42-), Phosphite(HPO32-) and Hypophosphite(HPO22-) together with the seven common anions. Since the contamination of the eluent by CO2 gas shortens the retention time, it is not appropriate to prepare the eluent from the concentrate solution which easily absorbs CO2 during storage. Please be sure to prepare the eluent from the powder reagent NaHCO3 each time and limit the period of use within 12 hours.


Sample : 20 μL
1. F- 2 mg/L
2. HPO22- 10 mg/L
3. Cl- 3 mg/L
4. NO2- 5 mg/L
5. Br- 10 mg/L
6. NO3- 10 mg/L
7. HPO42- 15 mg/L
8. HPO32- 10 mg/L
9. SO42- 10 mg/L
10. Oxalic acid 10 mg/L


Column       : Shodex IC SI-90 4E (4.0 mm I.D. x 250 mm)
Eluent       : 12 mM NaHCO3 aq.
Flow rate    : 1.2 mL/min
Detector     : Suppressed conductivity
Column temp. : 25 °C

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