Poly(vinyl alcohol) gel with different hydrophobicity are packed into Asahipak GF-310 HQ and GS-320 HQ columns. The hydrophobicity of the gel of the two columns is going stronger according to the temperature rising. Such influence is observed stronger with GF-310 than GS-320. Then under using water as the eluent at 50 °C, PEG is not eluted according to its molecular weight as shown the figure. In order to avoid such influence of hydrophobicity, it is applicable to make close the hydrophobicity of gel and eluent. For example the addition of organic solvent, such as Methanol, to eluent is in effect.
Please refer to Hydrophobicity of Columns.
Sample :
1. PEG, Poly(ethylene glycol) (MW: 20,000)
2. PEG, Poly(ethylene glycol) (MW: 4,000)
3. PEG, Poly(ethylene glycol) (MW: 1,000)
4. PEG, Poly(ethylene glycol) (MW: 400)
5. EG, Ethylene glycol (MW: 62)
Columns : Shodex Asahipak GF-310 HQ or GS-320 HQ (7.5 mm I.D. x 300 mm each) Eluent : H2O Flow rate : 0.5 mL/min Detector : RI Column temp. : 30 °C or 40 °C or 50 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Pullulan Standards (1) (SB-804 HQ)
- Pullulan Standards (2) (GF-310 HQ)
- Pullulan Standards (4) (Effects of Flow Rate) (SB-2002.5)
- Pullulan Standards (5) (Effects of Temperature) (SB-2002.5)
- Pullulan Standards (7) (GS-220 HQ)
- Pullulan Standards (9) (GF-7M HQ)
- Poly(Ethylene Oxide) and Poly(Ethylene Glycol) Standards (SB-804 HQ)
- Dextran (1) (SB-805 HQ)
- Dextran (2) (DMSO Eluent) (KF-806M)
- Dextran (3) (SB-806M HQ)
- Polyacrylamide (1) (SB-805 HQ)
- Polyacrylamide (3) (Comparison with SB-807 HQ and SB-806 HQ)
- Poly(Vinyl Alcohol) (2) (Aqueous Eluent) (SB-806 HQ + SB-803 HQ)
- Sodium Polyacrylate (1) (SB-803 HQ + SB-802 HQ)
- Optimization of Mobile Phase for the Analysis of Sodium Polyacrylate (GF-7M HQ)
- Polyvinylpyrrolidone (3) (NaCl aq./CH3CN Eluent) (SB-806M HQ)
- Polyvinylpyrrolidone (6) (NaCl aq./CH3CN Eluent) (SB-806M HQ)
- Sodium Poly(Styrene Sulfonate) (1) (GF-510 HQ)
- Sodium Poly(Styrene Sulfonate) (2) (GF-7M HQ)
- Selection of Column (1) (Sodium Poly(Styrene Sulfonate))
- Selection of Column (2) (Sodium Polyacrylate)
- Selection of Column (3) (Poly(Sodium Methacrylate))
- Poly(Ethylene Glycol) Standards (6) (DE-613)
- Poly(Ethylene Glycol) Standards (7) (SB-2002.5)
- Poly(Ethylene Glycol) Standards (8) (SB-802.5 HQ)
- Sodium Dextran Sulfate (SB-806M HQ)
- Poly(Ethylene Glycol) Standards (9) (GS-220 HQ)
- Comparison of Chromatogram of PEG Mixtures
- Influence of Salt Concentration on Retention Time of PEG with GF-310 HQ Column
- Effects of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent
- Effects of Analysis of PEG Mixtures with GS-320 HQ by Composition of Eluent
- Colominic Acid (SB-804 HQ)
- SEC Analysis of Latex Particles (KW405-4F)
- Poly(Sodium Methacrylate) (GF-7M HQ)
Applications (Related Information)
- SEC Analysis of Ionic Sample
- Calibration Curves for SB-800 HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for SB-800 HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves for SB-806M HQ (Aqueous Eluent: Pullulan, PEG and PEO)
- Calibration Curve for SB-807 HQ (Aqueous Eluent: Pullulan)
- Calibration Curves for LB-800 Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GF-HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GF-HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves for GS-HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GS-HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves of Hydrophilic Polymers (1) (Sodium Poly(Styrene Sulfonate))
- Calibration Curves of Hydrophilic Polymers (2) (Sodium Polyacrylate)
- Calibration Curves of Hydrophilic Polymers (3) (Poly(Sodium Methacrylate))