The comparison of chromatograms of mixture of PEG(MW: 20000, 4000, 1000, 400) and Ethyrene glycol(EG) with Asahipak GS-320 HQ column depending on the composition ratio of water and methanol is shown Fig.1. Since GS-320 HQ has higher polarity than GF-310 HQ, with GS-320 HQ, even if it uses 100 % of water for eluent, hydrophobic adsorption does not take place and PEG are separated in SEC mode.
Please refer to Effects of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent .
Sample : 0.1 % each, 100 μL
1. Poly(ethylene glycol) (Mw: 20,000)
2. Poly(ethylene glycol) (Mw: 4,000)
3. Poly(ethylene glycol) (Mw: 1,000)
4. Poly(ethylene glycol) (Mw: 400)
5. EG, Ethylene glycol
Please refer to Effects of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent .

Sample : 0.1 % each, 100 μL
1. Poly(ethylene glycol) (Mw: 20,000)
2. Poly(ethylene glycol) (Mw: 4,000)
3. Poly(ethylene glycol) (Mw: 1,000)
4. Poly(ethylene glycol) (Mw: 400)
5. EG, Ethylene glycol

Column : Shodex Asahipak GS-320 HQ (7.5 mm I.D. x 300 mm) Detector : RI Column temp. : 30 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Pullulan Standards (1) (SB-804 HQ)
- Pullulan Standards (2) (GF-310 HQ)
- Pullulan Standards (4) (Effects of Flow Rate) (SB-2002.5)
- Pullulan Standards (5) (Effects of Temperature) (SB-2002.5)
- Pullulan Standards (7) (GS-220 HQ)
- Pullulan Standards (9) (GF-7M HQ)
- Poly(Ethylene Oxide) and Poly(Ethylene Glycol) Standards (SB-804 HQ)
- Dextran (1) (SB-805 HQ)
- Dextran (2) (DMSO Eluent) (KF-806M)
- Dextran (3) (SB-806M HQ)
- Polyacrylamide (1) (SB-805 HQ)
- Polyacrylamide (3) (Comparison with SB-807 HQ and SB-806 HQ)
- Poly(Vinyl Alcohol) (2) (Aqueous Eluent) (SB-806 HQ + SB-803 HQ)
- Sodium Polyacrylate (1) (SB-803 HQ + SB-802 HQ)
- Optimization of Mobile Phase for the Analysis of Sodium Polyacrylate (GF-7M HQ)
- Polyvinylpyrrolidone (3) (NaCl aq./CH3CN Eluent) (SB-806M HQ)
- Polyvinylpyrrolidone (6) (NaCl aq./CH3CN Eluent) (SB-806M HQ)
- Sodium Poly(Styrene Sulfonate) (1) (GF-510 HQ)
- Sodium Poly(Styrene Sulfonate) (2) (GF-7M HQ)
- Selection of Column (1) (Sodium Poly(Styrene Sulfonate))
- Selection of Column (2) (Sodium Polyacrylate)
- Selection of Column (3) (Poly(Sodium Methacrylate))
- Poly(Ethylene Glycol) Standards (6) (DE-613)
- Poly(Ethylene Glycol) Standards (7) (SB-2002.5)
- Poly(Ethylene Glycol) Standards (8) (SB-802.5 HQ)
- Sodium Dextran Sulfate (SB-806M HQ)
- Poly(Ethylene Glycol) Standards (9) (GS-220 HQ)
- Influence of Column Temperature on Retention Time of PEG (GF-310 HQ and GS-320 HQ)
- Comparison of Chromatogram of PEG Mixtures
- Influence of Salt Concentration on Retention Time of PEG with GF-310 HQ Column
- Effects of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent
- Colominic Acid (SB-804 HQ)
- SEC Analysis of Latex Particles (KW405-4F)
- Poly(Sodium Methacrylate) (GF-7M HQ)
Applications (Related Information)
- SEC Analysis of Ionic Sample
- Calibration Curves for SB-800 HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for SB-800 HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves for SB-806M HQ (Aqueous Eluent: Pullulan, PEG and PEO)
- Calibration Curve for SB-807 HQ (Aqueous Eluent: Pullulan)
- Calibration Curves for LB-800 Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GF-HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GF-HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves for GS-HQ Series (Aqueous Eluent: Pullulan)
- Calibration Curves for GS-HQ Series (Aqueous Eluent: PEG, PEO)
- Calibration Curves of Hydrophilic Polymers (1) (Sodium Poly(Styrene Sulfonate))
- Calibration Curves of Hydrophilic Polymers (2) (Sodium Polyacrylate)
- Calibration Curves of Hydrophilic Polymers (3) (Poly(Sodium Methacrylate))