Influence of Salt Concentration on Retention Time of PEG with GF-310 HQ Column

If water solution containing salt is used as eluent, PEG will be absorbed to Asahipak GF-310 HQ, because hydrophobic interaction of PEG will be strong. This influence will becomel stronger if column temperature is made high.


Sample :
1. PEG, Poly(ethylene glycol) (MW: 4,000)
2. PEG, Poly(ethylene glycol) (MW: 1,000)
3. EG, Ethylene glycol (MW: 62)


Column       : Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm)
Eluent       : H2O or 0.1 M NaCl aq.
Flow rate    : 0.5 mL/min
Detector     : RI
Column temp. : 30 °C or 40 °C

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