Polyacrylamide was analyzed with OHpak SB-806 HQ (exclusion limit: 2.0x10⁷ g/mol) and SB-807 HQ (exclusion limit: 5.0x10⁸ g/mol). In the analysis with SB-806 HQ, polyacrylamide elutes around exclusion limit. On the other hand, in the analysis with SB-807 HQ, polyacrylamide elutes with SEC mode.


Sample : 100 μL
Polyacrylamide

Columns      : Shodex OHpak SB-807 HQ, SB-806 HQ (8.0 mm I.D. x 300 mm each)
Eluent       : 0.2 M NaCl aq.
Flow rate    : 0.5 mL/min
Detector     : RI and MALS(Multi angle light scattering detector)
Column temp. : 30 °C