In case of polymers which have both hydrophobic and hydrophillic groups, hydrophobic interaction with packing material may exist. For such polymers, the addition of organic solvents in the eluent can remove hydrohorbic interatction and realize pure SEC mode. Since an Aqueous/Organic (multi-solvent use) SEC column, Asahipak GF-7M HQ can be used with many kinds organic solvents, it is suited to be used with mixed solvents.


Sample : 50 µL
Sodium poly(styrene sulfonate)
Mw : 990,000 0.1 %
Mw : 149,000 0.2 %
Mw : 48,600 0.2 %
Mw : 16,800 0.2 %
Mw : 4,300 0.2 %

Column       : Shodex Asahipak GF-7M HQ (7.5 mm I.D. x 300 mm)
Eluent       : 50 mM LiCl aq./CH3CN=60/40
Flow rate    : 0.6 mL/min
Detector     : RI
Column temp. : 30 °C