Polymer-based amino column, Asahipak NH2P-50 4E, was used to analyze water-soluble vitamins under HILIC mode. Six water-soluble vitamins were separated within 5 minutes.
- [Note]
- This analysis requires column equilibration.
For column equilibration: First introduce 60 mM phosphoric acid at 0.5 mL/min for 2 hours, and then switch to the eluent.
Sample: 20 μL
- 1.Vitamin B6 50 μg/mL
- 2.Nicotinamide 10 μg/mL
- 3.Vitamin B12 10 μg/mL
- 4.Nicotinic acid 10 μg/mL
- 5.Folic acid 10 μg/mL
- 6.Vitamin C 10 μg/mL
- Column
- ：Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
- Eluent
- ：40 mM H3PO4 aq./CH3CN=45/55
- Flow rate
- ：1.0 mL/min
- Detector
- ：UV (254 nm)
- Column temp.
- ：40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Water-Soluble Vitamins (1) (DE-413)
- Water-Soluble Vitamins (4) (DM-614)
- Simultaneous Analysis of Water-Soluble Vitamins (DE-413)
- LC/MS Analysis of Water-soluble Vitamins (DE-213)
- Vitamin B6 (1) (ODP-50 6D)
- Vitamin B12 (SB-802.5 HQ)
- Vitamin C (1) (KC-811)
- Vitamin C (3) (NH2P-50 4E)
- Soft Drink Containing Vitamin C (NH2P-50 4E)
- LC/MS Analysis of Pantothenic Acid and Vitamin C (VT-50 2D)
- Separation of Ascorbic Acid and Isoascorbic Acid (DE-413)
- Analysis of Ascorbic Acid Injection According to USP-NF Method (DM-614)
- Carnitine (NN-814)
- LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)
- Food Containing Vitamins (DE-413)
- Nutrient Beverage (DE-413)
- Separation of Water-Soluble Vitamin and Sulfite (KC-811)
- Vitamin D (ODP-50 6E)
- Margarine (KF-801)
- Fat-Soluble Vitamins (1) (ODP-50 4E)
- Fat-Soluble Vitamins (3) (KF-801)
- Carotene Analysis in Carrot Juice (C18U 2D)