Vitamins are essential components for maintaining physiological functions in living bodies. In particular, five water-soluble essential vitamins, B1, B2, C, nicotinic acid and nicotinamide are sometimes lacking in human bodies. The necessary quantities of three water-soluble vitamins, B6, B12 and folic acid have been determined by the NRC (National Research Council), though these vitamins are seldom lacking in human bodies. This chromatogram shows an example of the analysis of such water-soluble vitamins except folic acid. This analysis has been performed by reversed phase mode with gradient elution. Good calibration curves are obtained for the vitamins using RSpak DE-413 (a column for reversed phase chromatography). As an example, calibration curves for vitamin B2 and vitamin B6 are shown below.
Sample : 20 μL
1. Vitamin C
2. Niacin
3. Vitamin B6
4. Niacinamide
5. Vitamin B1
6. Vitamin B12
7. Vitamin B2
Column : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm) Eluent : (A); 1/30 M Potasium phosphate buffer (pH6.8)/CH3CN=98/2 (B); H2O/CH3CN=50/50 0 min to 4 min, 100 % (A) 4 min to 15 min, 100 % (A) to 30 % (A) after 15 min, 30 %(A) Flow rate : 0.6 mL/min Detector : UV (254 nm) Column temp. : 35 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Water-Soluble Vitamins (4) (DM-614)
- Water-Soluble Vitamins (5) (NH2P-50 4E)
- Simultaneous Analysis of Water-Soluble Vitamins (DE-413)
- LC/MS Analysis of Water-soluble Vitamins (DE-213)
- Vitamin B6 (1) (ODP-50 6D)
- Vitamin B12 (SB-802.5 HQ)
- Vitamin C (1) (KC-811)
- Vitamin C (3) (NH2P-50 4E)
- Soft Drink Containing Vitamin C (NH2P-50 4E)
- LC/MS Analysis of Pantothenic Acid and Vitamin C (VT-50 2D)
- Separation of Ascorbic Acid and Isoascorbic Acid (DE-413)
- Analysis of Ascorbic Acid Injection According to USP-NF Method (DM-614)
- Carnitine (NN-814)
- LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)
- Food Containing Vitamins (DE-413)
- Nutrient Beverage (DE-413)
- Separation of Water-Soluble Vitamin and Sulfite (KC-811)
- Vitamin D (ODP-50 6E)
- Margarine (KF-801)
- Fat-Soluble Vitamins (1) (ODP-50 4E)
- Fat-Soluble Vitamins (3) (KF-801)
- Carotene Analysis in Carrot Juice (C18U 2D)