Vitamin A palmitate was analyzed using GPC KF-801. Wave length of 325 nm at which it has maximum UV absorption was used for the detection. Usually, saponification is necessary to analyze vitamin A as the sample pretreatment. However, in this analysis, no complicated sample pretreatment procedure was necessary. What is necessary is just to dissolve the sample in the eluent (THF) and filtrate it using a 0.45 μm disposable filter. This method using KF-801 is a very simple way to analyze vitamin A.


Sample : Margarine 0.2 %, 100 μL
1. Vitamin A palmitate

Columns      : Shodex GPC KF-801 (8.0 mm I.D. x 300 mm) x 2
Eluent       : THF
Flow rate    : 1.0 mL/min
Detector     : UV (325 nm)
Column temp. : 40 °C