According to the United States Pharmacopeia and the National Formulary (USP 40-NF 35*), analysis of ascorbic acid should be carried out with a column with L39 packing material and meets following requirements. The RSpak DM-614 confirmed the requirements were met.

System suitability requirements:
   Tailing factor: ≤ 1,6
   Theoretical plate number: ≥ 3,500
   Relative standard deviation (RSD): ≤ 1.5%

*The version at the time of the application acquisition. 


Sample : 4 μL
1. Ascorbic acid 0.05 %

Column       : Shodex RSpak DM-614 (6.0 mm I.D. x 150 mm)
Eluent       : 0.055 M Na2HPO4 + 0.045 M KH2PO4 aq. (adjusted pH 2.5 with H3PO4)
Flow rate    : 0.6 mL/min
Detector     : UV (245 nm)
Column temp. : 30 °C