Analysis of Ascorbic Acid Injection According to USP-NF Method (DM-614)

According to the United States Pharmacopeia and the National Formulary (USP 40-NF 35*), analysis of ascorbic acid should be carried out with a column with L39 packing material and meets following requirements. The RSpak DM-614 confirmed the requirements were met.

System suitability requirements

Tailing factor: ≤ 1.6
Theoretical plate number: ≥ 3,500
Relative standard deviation (RSD): ≤ 1.5%

*The version at the time of the application acquisition.

Sample: 4 μL

  1. 1.Ascorbic acid 0.05 %
Column
Shodex RSpak DM-614 (6.0 mm I.D. x 150 mm)
Eluent
0.055 M Na2HPO4 + 0.045 M KH2PO4 aq. (adjusted pH2.5 with H3PO4 aq.)
Flow rate
0.6 mL/min
Detector
UV (245 nm)
Column temp.
30 ℃

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