Soft Drink Containing Vitamin C (NH2P-50 4E)

Vitamin C in soft drink was analyzed using Asahipak NH2P-50 4E.

[Note] This analysis requires column equilibration.
For column equilibration: First introduce 60 mM Phosphoric acid aq. at 0.5 mL/min for 2 hours, and then switch to the eluent.

Sample pretreatment

  1. 1.Add 5 % methaphosphoric acid aq. to make up 30 mL sample solution.
  2. 2.Filtrate using a 0.45 μm disposable filter.
  3. 3.Just before the injection into HPLC, add purified water to make 10-fold solution.
    (The concentration of methaphosphoric acid should be 0.5 % or lower.)

Sample: 10 µL
Soft drink containing Vitamin C

Quantification result Scroll
No. Substance Concentration (μg/mL) Contents in100 mL (mg)
1  L-Ascorbic acid 37.2 372
Column
：Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
：20 mM NaH2PO4 + 30 mM H3PO4 (pH2.2)/CH3CN=20/80
Flow rate
：1.0 mL/min
Detector
：UV (254 nm)
Column temp.
：35 ℃

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