LC/MS Analysis of Water-soluble Vitamins (DE-213)

Ten kinds of water-soluble vitamins were simultaneously analyzed using RSpak DE-213, a polymer-based reversed phase chromatography.


Sample : 5 μL
Vitamin B1 10 µM
Vitamin C 100 µM
Vitamin B6 10 µM
Vitamin B3 10 µM
Nicotinic acid amide 10 µM
Vitamin B5 10 µM
Vitamin B12 10 µM
Folic acid 2 µM
Vitamin B2 10 µM
Vitamin B7 10 µM
(in 50 mM HCOOH aq.)


Column       : Shodex RSpak DE-213 (2.0 mm I.D. x 150 mm)
Eluent       : (A); H2O/0.5 M HCOOH aq./0.5 M HCOONH4 aq.=96/3/1
               (B); H2O/0.5 M HCOOH aq./0.5 M HCOONH4 aq./CH3CN=46/3/1/50
               Linear gradient; (B %) 0 to 100 % (0 to 5 min), 100 % (5 to 10 min)
Flow rate    : 0.2 mL/min
Detector     : ESI-MS (SIM)
Column temp. : 30 °C

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Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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