Fat-Soluble Vitamins (1) (ODP-50 4E)

Asahipak ODP-50 4E was used for the simultaneous analysis of fat-soluble vitamins. Good calibration curves were obtained for them.


Sample : 20 μL
1. Vitamin K3 1.5 μg/mL
2. Vitamin A 1.0 IU/mL (0.3 μg/mL)
3. Vitamin A acetate 0.5 IU/mL (1.9 μg/mL)
4. Vitamin D2 13.2 IU/mL (0.3 μg/mL)
5. Vitamin D3 13.2 IU/mL (0.3 μg/mL)
6. Vitamin E acetate 2.4 μg/mL
7. Vitamin E 2.5 μg/mL
8. Vitamin K1 2.4 μg/mL



Column       : Shodex Asahipak ODP-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : CH3CN/CH3OH=50/50
Flow rate    : 0.6 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C

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