Food Containing Vitamins (DE-413)

Vitamins in food were analyzed using RSpak DE-413, a column for reversed phase chromatography.

Sample pretreatment

  1. 1.Dissolve 80 mg of the food containing vitamins in 12 mL of the eluant (A).
  2. 2.Pass through a 0.45 μm filter before injecting into HPLC.

Sample: 20 μL

Quantitative resultsScroll
No. Substances Concentration (μg/mL) Contents (mg) in 4 g
1 Vitamin C 145.8 87.5
2 Vitamin B6   21.2 12.7
3 Vitamin B2     7.3   4.4
Column
：Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
Eluent
：(A); 1/30 M Potasium phosphate buffer (pH6.8)/CH3CN=98/2
(B); H2O/CH3CN=50/50
Gradient; 0 min to 4 min, 100 % (A), 4 min to 15 min, 100 % (A) to 30 % (A), After 15 min, 30 % (A)
Flow rate
：0.6 mL/min
Detector
：UV (254 nm)
Column temp.
：35 ℃

Sample Name Index

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Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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