Ascorbic acid and isoascorbic acid were separatd using two columns of RSpak DE-413 (a column for polymer-based reversed phase chromatography). The calibration curve of ascorbic acid and isoascorbic acid shows good linearity respectively in the range from 1 to 100 μg/mL.
Sample : 10 μL
5 μg/mL each (in 0.1 % metaphosphoric acid)
1. Ascorbic acid
2. Isoascorbic acid 
Column : Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm) x 2 Eluent : 10 mM H3PO4 aq. Flow rate : 0.6 mL/min Detector : UV (254 nm) Column temp. : 40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Water-Soluble Vitamins (1) (DE-413)
- Water-Soluble Vitamins (4) (DM-614)
- Water-Soluble Vitamins (5) (NH2P-50 4E)
- Simultaneous Analysis of Water-Soluble Vitamins (DE-413)
- LC/MS Analysis of Water-soluble Vitamins (DE-213)
- Vitamin B6 (1) (ODP-50 6D)
- Vitamin B12 (SB-802.5 HQ)
- Vitamin C (1) (KC-811)
- Vitamin C (3) (NH2P-50 4E)
- Soft Drink Containing Vitamin C (NH2P-50 4E)
- LC/MS Analysis of Pantothenic Acid and Vitamin C (VT-50 2D)
- Analysis of Ascorbic Acid Injection According to USP-NF Method (DM-614)
- Carnitine (NN-814)
- LC/MS Analysis of Carnitine and Trimethyl Glycine (NH2P-40 2D)
- Food Containing Vitamins (DE-413)
- Nutrient Beverage (DE-413)
- Separation of Water-Soluble Vitamin and Sulfite (KC-811)
- Vitamin D (ODP-50 6E)
- Margarine (KF-801)
- Fat-Soluble Vitamins (1) (ODP-50 4E)
- Fat-Soluble Vitamins (3) (KF-801)
- Carotene Analysis in Carrot Juice (C18U 2D)