The characteristic analysis of sugar chains bound to proteins is essential for the development of antibody drugs which has recently received attention. Various derivatization reagents are used for high sensitivity analysis of sugar chains with HPLC, and 2-aminobenzoic acid (2-AA) is one of typical sugar derivatization reagents. In this application, 2-AA Labeled sugar chain (glucose homopolymer) was separated usign a polymer-based amino column, under HILIC mode. Derivatization with 2 - AA allows the use of a fluorescence detector for sugar chain analysis, and G1 (glucose) can be detected in amol levels.
Sample : 50 μL
2-AA Labeled glucose homopolymer ladder (2-AA Labeled dextran hydrolysate ladder)
10 nM (G1 conc.)
Column : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm) Eluent : (A); 2 % CH3COOH in CH3CN (B); 5 % CH3COOH + 3 % Triethylamine in H2O (B %) 30 % to 95 % (2 to 82 min) Flow rate : 1.0 mL/min Detector : Fluorescence (Ex.350 nm, Em.425 nm) Column temp. : 40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
- Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
- LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG) (NH2P-40 2D)
- LC/MS/MS Analysis of Saccharides (NH2P-40 2D)
- LC/MS/MS Analysis of Saccharides (VG-50 2D)
- LC/MS Analysis of Phosphorylated Saccharides (VT-50 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)
- LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)