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LC/MS/MS Analysis of Saccharides (VG-50 2D)

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HILICpak VG-50 2D, a polymer based amino column, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, and is effective for high sensitive detection.


Sample : 50ng/mL each
(in H2O/CH3CN=1/9), 5μL
meso-Erythritol
Arabinose
Xylose
Fructose
Mannose
Glucose
Sucrose
Lactose
Maltose


Column       : Shodex HILICpak VG-50 2D (2.0mmI.D. x 150mm)
Eluent       : (A); 0.1% NH3 aq. (B); CH3CN
               Linear gradient ; (B%) 95% (0 to 5min),
                                      95% to 80% (5 to 15min), 80% (15 to 20min)
Flow rate    : 0.3mL/min
Detector     : ESI-MS/MS (MRM Negative)
Column temp. : 60°C

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