HILICpak VG-50 2D, a polymer based amino column, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, and is effective for high sensitive detection.


Sample : 50 ng/mL each
(in H₂O/CH₃CN=1/9), 5 μL
meso-Erythritol
Arabinose
Xylose
Fructose
Mannose
Glucose
Sucrose
Lactose
Maltose

Column       : Shodex HILICpak VG-50 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A); 0.1 % NH3 aq. (B); CH3CN
               Linear gradient ; (B %) 95 % (0 to 5 min),
                                      95 % to 80 % (5 to 15 min), 80 % (15 to 20 min)
Flow rate    : 0.3 mL/min
Detector     : ESI-MS/MS (MRM Negative)
Column temp. : 60 °C