HILICpak VG-50, a polymer-based amino column, is suitable for sugar analysis and it is also is available for simultaneous analysis of organic acids and amino acids with LC/MS detection under alkaline conditions. High anionic substances can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.
Sample: 1μg/mL each (in H2O/CH3CN=1/4), 5μL

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Amino acids | ||||
---|---|---|---|---|
Leu | Ile | Phe | Met | Val |
Pro | Trp | Ala | Thr | Gly |
Ser | Lys | Gln | Asn | His |
Arg | Tyr | Glu | Asp | Cys |
Organic acids | ||||
Pyruvic acid | Lactic acid | Oxalic acid | Glyceric acid | Malic acid |
Tartaric acid | Malonic acid | Maleic acid | Citric acid | |
Saccharides and Sugar alcohols | ||||
meso-Erythritol | Arabinose | Xylose | N-Acetylglucosamine | Fructose |
Mannose | Glucose | Glucosamine | Sucrose | Lactose |
Maltose | Raffinose | Glucuronic acid | Galacturonic acid |
- Column
- :Shodex HILICpak VG-50 2D (2.0 mm I.D. x 150 mm)
- Eluent
- :(A); 0.5 % NH3 aq. (B); CH3CN
Linear gradient (High pressure);
(B %) 80 % (0 to 2min), 80 % to 10 % (2 to 12 min), 10 % (12 to 15 min), 80 % (15 to 20 min) - Flow rate
- :0.2 mL/min
- Detector
- :ESI-MS (SIM Negative)
- Column temp.
- :40 ℃
Sample Name Index
- meso-Erythritol
- Alanine, Ala
- Arabinose
- Arginine, Arg
- Asparagine, Asn
- Aspartic acid, Asp
- Citric acid
- Cysteine, Cys
- D-Glucosamine
- D-Glucuronic acid
- Fructose
- Galacturonic acid
- Glucose
- Glutamic acid, Glu
- Glutamine, Gln
- Glyceric acid
- Glycine, Gly
- Histidine, His
- Isoleucine, Ile
- Lactic acid
- Lactose
- Leucine, Leu
- Lysine, Lys
- Maleic acid
- Malic acid
- Malonic acid
- Maltose
- Mannose
- Methionine, Met
- N-Acetyl-D-glucosamine
- Oxalic acid
- Phenylalanine, Phe
- Proline, Pro
- Pyruvic acid
- Raffinose
- Serine, Ser
- Sucrose, Saccharose
- Tartaric acid
- Threonine, Thr
- Tryptophan, Trp
- Tyrosine, Tyr
- Valine, Val
- Xylose
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
- Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
- LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG) (NH2P-40 2D)
- Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
- LC/MS/MS Analysis of Saccharides (NH2P-40 2D)
- LC/MS/MS Analysis of Saccharides (VG-50 2D)
- LC/MS Analysis of Phosphorylated Saccharides (VT-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)
- LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)