Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)

HILICpak VG-50, a polymer-based amino column, is suitable for sugar analysis and it is also is available for simultaneous analysis of organic acids and amino acids with LC/MS detection under alkaline conditions. High anionic substances can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.

Sample: 1μg/mL each (in H2O/CH3CN=1/4), 5μL

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Amino acids
Leu Ile Phe Met Val
Pro Trp Ala Thr Gly
Ser Lys Gln Asn His
Arg Tyr Glu Asp Cys
Organic acids
Pyruvic acid Lactic acid Oxalic acid Glyceric acid Malic acid
Tartaric acid Malonic acid Maleic acid Citric acid 　
Saccharides and Sugar alcohols
meso-Erythritol Arabinose Xylose N-Acetylglucosamine Fructose
Mannose Glucose Glucosamine Sucrose Lactose
Maltose Raffinose Glucuronic acid Galacturonic acid
Column
：Shodex HILICpak VG-50 2D (2.0 mm I.D. x 150 mm)
Eluent
：(A); 0.5 % NH3 aq. (B); CH3CN
Linear gradient (High pressure);
(B %) 80 % (0 to 2min), 80 % to 10 % (2 to 12 min), 10 % (12 to 15 min), 80 % (15 to 20 min)
Flow rate
：0.2 mL/min
Detector
：ESI-MS (SIM Negative)
Column temp.
：40 ℃

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