Analysis of glycan has many challenges since even the purified protein contains non-uniform glycans and the analysis requires identifying branched structures and isomers specific to glycans. HPLC analysis is an effective method to solve those problems. Various glycans had been identified and quantified using two-dimensional liquid chromatography combining affinity and HILIC modes as their separation. The application below shows the separation of 2-Aminobenzoic acid (2AA) labeled glycans. Asahipak NH2P-40 2D, a polymer-based amino HILIC column, was used with an ESI-TOF-MS to identify the glycans.
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.

Sample : 2 μL
Human IgG (1 μg protein)
2AA-labeled N-Glycan

*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.

Sample : 2 μL
Human IgG (1 μg protein)
2AA-labeled N-Glycan

Column : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm) Eluent : (A); 95 % MeCN/0.1 % Formic acid (B); 5 % MeCN/0.1 % Formic acid Linear gradient; (B %) 30 % (0 - 2.5 min), 30 - 95 % (2.5 - 20 min) Flow rate : 0.2 mL/min Detector : ESI-TOF MS (Polarity : Negative, Full MS range : 2000, CDL temp.: 190 °C, Detector voltage: 1.7 kV, Neuburising gas: 1.5 L/min) Column temp. : 45 °CData provided by Dr. Mitsuhiro Kinoshita, School of Pharmacy, Kinki University
Sample Name Index
Applications
- Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
- Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
- Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
- LC/MS/MS Analysis of Saccharides (NH2P-40 2D)
- LC/MS/MS Analysis of Saccharides (VG-50 2D)
- LC/MS Analysis of Phosphorylated Saccharides (VT-50 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)
- LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)