LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)

Sugar analysis column, Asahipak NH2P-40 2D, was used with a LC/MS to separate N-Acetylchitooligosaccharides. By adding ammonia to the solvent, it enhances the ionization of saccharides, and thus provides high sensitivity detection.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.

Sample: 5 μL
Mixture of N-Acetylchitooligosaccharides (Seikagaku Co. code:800104 Chitooligosaccharides Mixture) 500 ng/mL

  1. 1.N-Acetyl-D-glucosamine
  2. 2.di-N-Acetylchitobiose
  3. 3.tri-N-Acetylchitotriose
  4. 4.tetra-N-Acetylchitotetraose
  5. 5.penta-N-Acetylchitopentaose
  6. 6.hexa-N-Acetylchitohexaose
Column
：Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent
：(A); 0.05 % Ammonia aq. (B); CH3CN
Linear gradient; (B %) 75 % (0 min) -> 50 % (10 min) -> 50 % (15 min)
Flow rate
：0.2 mL/min
Detector
：ESI-MS (SIM Negative)
Column temp.
：30 ℃

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