Sugar analysis column, Asahipak NH2P-40 2D, was used with a LC/MS to separate N-Acetylchitooligosaccharides. By adding ammonia to the solvent, it enhances the ionization of saccharides, and thus provides high sensitivity detection.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.
Sample: 5 μL
Mixture of N-Acetylchitooligosaccharides (Seikagaku Co. code:800104 Chitooligosaccharides Mixture) 500 ng/mL
- 1.N-Acetyl-D-glucosamine
- 2.di-N-Acetylchitobiose
- 3.tri-N-Acetylchitotriose
- 4.tetra-N-Acetylchitotetraose
- 5.penta-N-Acetylchitopentaose
- 6.hexa-N-Acetylchitohexaose

- Column
- :Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
- Eluent
- :(A); 0.05 % Ammonia aq. (B); CH3CN
Linear gradient; (B %) 75 % (0 min) -> 50 % (10 min) -> 50 % (15 min) - Flow rate
- :0.2 mL/min
- Detector
- :ESI-MS (SIM Negative)
- Column temp.
- :30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
- Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
- LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG) (NH2P-40 2D)
- Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
- LC/MS/MS Analysis of Saccharides (NH2P-40 2D)
- LC/MS/MS Analysis of Saccharides (VG-50 2D)
- LC/MS Analysis of Phosphorylated Saccharides (VT-50 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)