Sugar analysis column, Asahipak NH2P-40 2D, was used with a LC/MS to separate N-Acetylchitooligosaccharides. By adding ammonia to the solvent, it enhances the ionization of saccharides, and thus provides high sensitivity detection.
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.
Sample : 5 μL, Mixture of N-Acetylchitooligosaccharides
(Seikagaku Co. code:800104 Chitooligosaccharides Mixture) 500 ng/mL
1. N-Acetyl-D-glucosamine
2. di-N-Acetylchitobiose
3. tri-N-Acetylchitotriose
4. tetra-N-Acetylchitotetraose
5. penta-N-Acetylchitopentaose
6. hexa-N-Acetylchitohexaose
Column : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm) Eluent : (A); 0.05 % Ammonia aq. (B); CH3CN Linear gradient; (B %) 75 % (0 min) -> 50 % (10 min) -> 50 % (15 min) Flow rate : 0.2 mL/min Detector : ESI-MS (SIM Negative) Column temp. : 30 °C
Sample Name Index
Applications
- Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
- Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
- LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG) (NH2P-40 2D)
- Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
- LC/MS/MS Analysis of Saccharides (NH2P-40 2D)
- LC/MS/MS Analysis of Saccharides (VG-50 2D)
- LC/MS Analysis of Phosphorylated Saccharides (VT-50 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)