LC/MS Analysis of Phosphorylated Saccharides (VT-50 2D)

Various phosphorylated saccharides are synthesized as intermediates in glycolytic pathway and gluconeogenesis. Four kinds of phosphorylate saccharides were analyzed by HILICpak VT-50 2D under the conditions of LC/MS. This analytical condition can be also used for other detectors like RI, ELSD, and corona CAD.

Sample: 5 μL
1 µM each

  1. 1.Glucose-6-phosphate (G6P)
  2. 2.Fructose-6-phosphate (F6P)
  3. 3.Glucose-1-phosphate (G1P)
  4. 4.Fructose-1-phosphate (F1P)
Column
Shodex HILICpak VT-50 2D (2.0 mm I.D. x 150 mm)
Eluent
25 mM HCOONH4 aq./CH3CN=80/20
Flow rate
0.3 mL/min
Detector
ESI-MS (SIM Negative : m/z 259)   
Column temp.
60 ℃

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Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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