Various phosphorylated saccharides are synthesized as intermediates in glycolytic pathway and gluconeogenesis. Four kinds of phosphorylate saccharides were analyzed by HILICpak VT-50 2D under the conditions of LC/MS. This analytical condition can be also used for other detectors like RI, ELSD, and corona CAD.
Sample: 5 μL
1 µM each
- 1.Glucose-6-phosphate (G6P)
- 2.Fructose-6-phosphate (F6P)
- 3.Glucose-1-phosphate (G1P)
- 4.Fructose-1-phosphate (F1P)
- Column
- ：Shodex HILICpak VT-50 2D (2.0 mm I.D. x 150 mm)
- Eluent
- ：25 mM HCOONH4 aq./CH3CN=80/20
- Flow rate
- ：0.3 mL/min
- Detector
- ：ESI-MS (SIM Negative : m/z 259)
- Column temp.
- ：60 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
- Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
- LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG) (NH2P-40 2D)
- Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
- LC/MS/MS Analysis of Saccharides (NH2P-40 2D)
- LC/MS/MS Analysis of Saccharides (VG-50 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)
- LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)
- LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)