Simultaneous Analysis of Hydrophilic Substances in Energy Drink with LC/MS (VG-50 2D)

HILICpak VG-50, a polymer-based amino column, is suitable for sugar analysis and it is also is available for simultaneous analysis of water-soluble vitamins, organic acids and amino acids with LC/MS detection under alkaline conditions. In this applications, hydrophilic substances in an energy drink were simultaneously analyzed. High anionic substances can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.


Sample : 2μL
100-fold diluted Energy drink with H₂O/CH₃CN=1/1
Caffeine
Nicotinamide
Riboflavin
Fructose
Glucose
Pyridoxine
Sucrose
Isoleucine
Phenylalanine
Threonine
Glutamic acid
Citric acid

Column       : Shodex HILICpak VG-50 2D (2.0mmI.D. x 150mm)
Eluent       : (A); 0.5% NH3 aq. (B); CH3CN
               Linear gradient (High pressure); 
               (B%) 75% to 10% (0 to 5min)
                    10% (5 to 15min)
Flow rate    : 0.2mL/min
Detector     : ESI-MS (SIM)
Column temp. : 40°C