A polymer based amino column, Asahipak NH2P-40 2D, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, thus providing high sensitivity detection.
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Sample : 50 ng/mL each, 5 μL
1. meso-Erythritol
2. Xylitol
3. Fructose
4. Glucose
5. Sucrose
6. Lactose
7. Maltose
8. Raffinose

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A); 0.05 % NH3 aq./(B); CH3CN
               Isocratic ; (B %) 75 %
Flow rate    : 0.2 mL/min
Detector     : ESI-MS/MS (MRM Negative)
               (m/z)
               121 -> 82 (-) for meso-Erythritol, 151 -> 59 (-) for Xylitol,
               179 -> 89 (-) for Fructose and Glucose, 341 -> 59 (-) for Sucrose,
               341 -> 161 (-) for Lactose and Maltose, 503 -> 89 (-) for Raffinose
Column temp. : 40 °C