A polymer based amino column, Asahipak NH2P-40 2D, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, thus providing high sensitivity detection.
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Sample : 50 ng/mL each, 5 μL
1. meso-Erythritol
2. Xylitol
3. Fructose
4. Glucose
5. Sucrose
6. Lactose
7. Maltose
8. Raffinose
Column : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm) Eluent : (A); 0.05 % NH3 aq./(B); CH3CN Isocratic ; (B %) 75 % Flow rate : 0.2 mL/min Detector : ESI-MS/MS (MRM Negative) (m/z) 121 -> 82 (-) for meso-Erythritol, 151 -> 59 (-) for Xylitol, 179 -> 89 (-) for Fructose and Glucose, 341 -> 59 (-) for Sucrose, 341 -> 161 (-) for Lactose and Maltose, 503 -> 89 (-) for Raffinose Column temp. : 40 °C
Sample Name Index
Applications
- Analysis of Pyridylaminated (PA) Monosaccharides (NH2P-50 4E)
- Analysis of Pyridylaminated Sugar Chains (NH2P-50 4E)
- LC/TOF-MS Analysis of 2-Aminobenzoic Acid Labeled N-Glycan (Human IgG) (NH2P-40 2D)
- Analysis of 2-Aminobenzoic Acid Labeled Glucose Homopolymer (NH2P-50 4E)
- LC/MS/MS Analysis of Saccharides (VG-50 2D)
- LC/MS Analysis of Phosphorylated Saccharides (VT-50 2D)
- Simultaneous Analysis of Saccharides, Organic Acids and Amino Acids with LC/MS (VG-50 2D)
- LC/MS Analysis of Glucose and Gluconic Acid (VG-50 2D)
- LC/MS Analysis of N-Acetylchitooligosaccharides (NH2P-40 2D)