LC/MS/MS Analysis of Saccharides (NH2P-40 2D)

A polymer based amino column, Asahipak NH2P-40 2D, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, thus providing high sensitivity detection.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.

Sample: 5 μL
50 ng/mL each

  1. 1.meso-Erythritol
  2. 2.Xylitol
  3. 3.Fructose
  4. 4.Glucose
  5. 5.Sucrose
  6. 6.Lactose
  7. 7.Maltose
  8. 8.Raffinose
Column
：Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent
：(A) 0.05 % NH3 aq./(B) CH3CN
Isocratic; (B %) 75 %
Flow rate
：0.2 mL/min
Detector
：ESI-MS/MS (MRM Negative)
(m/z)
121→82(-) for meso-Erythritol, 151→59(-) for Xylitol, 179→89(-) for Fructose and Glucose,
341→59(-) for Sucrose, 341→161(-) for Lactose and Maltose, 503→89(-) for Raffinose
Column temp.
：40 ℃

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