LC/MS/MS Analysis of Saccharides (NH2P-40 2D)

A polymer based amino column, Asahipak NH2P-40 2D, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the eluent enhances the ionization of saccharides, thus providing high sensitivity detection.


Sample : 50ng/mL each, 5μL
1. meso-Erythritol
2. Xylitol
3. Fructose
4. Glucose
5. Sucrose
6. Lactose
7. Maltose
8. Raffinose

Column       : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)
Eluent       : (A) 0.05% NH3 aq. /(B) CH3CN
               Isocratic ; (B%) 75%
Flow rate    : 0.2mL/min
Detector     : ESI-MS/MS (MRM Negative)
               (m/z)
               121→82(-) for meso-Erythritol, 151→59(-) for Xylitol,
               179→89(-) for Fructose and Glucose, 341→59(-) for Sucrose,
               341→161(-) for Lactose and Maltose, 503→89(-) for Raffinose
Column temp. : 40°C