Pyridylaminated (PA) monosaccharides were analysed by Asahipak NH2P-50 4E. Carbohydrates are usually detected by refractive index detector (RI), but pyridylamination of saccharides enables high sensitivity analysis by fluorescence detector.


Sample : 5 μL
1. 2-Aminopyridine 0.1 pmol/μL
2. PA-Rhamnose 1 pmol/μL
3. PA-Fucose 1 pmol/μL
4. PA-Ribose 1 pmol/μL
5. PA-Xylose 1 pmol/μL
6. PA-N-Acetylglucosamine 1 pmol/μL
7. PA-N-Acetylgalactosamine 1 pmol/μL
8. PA-Mannose 1 pmol/μL
9. PA-Glucose 1 pmol/μL
10. PA-Galactose 1 pmol/μL

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : Phosphoric acid/H2O/CH3CN=1/14/85
Flow rate    : 0.5 mL/min
Detector     : Fluorescence (Ex. 310 nm, Em. 380 nm)
Column temp. : 40 °C