A method was optimized for the simultaneous analysis of anionic and non-ionic surfactants using an Aqueous/Organic (multi-solvent use) SEC column, Asahipak GF-210 HQ. Polyvinyl alcohol used for GF-210 HQ packed gel is slightly negatively charged. By using mobile phase without salt retains this negatively charged surface characteristic; this results in the fast elution of anionic surfactant by ion exclusion effect, and thus separates it from non-ionic surfactant. Meanwhile, addition of salt to the mobile phase suppresses the ion exclusion effect and retains the anionic surfactant, thus it is not separated from the non-ionic surfactant.
Sample: 50 μL
- 1.Sodium p-n-octylbenzenesulfonate 100 mg/L
- 2.Triton X-114, (Polyoxyethylene(8) octylphenyl ether) 1000 mg/L
- Column
- :Shodex Asahipak GF-210 HQ (7.5 mm I.D. x 300 mm)
- Eluent
- :(Left); H2O/CH3CN=65/35 (Right); 50 mM NaCl aq./CH3CN=65/35
- Flow rate
- :0.8 mL/min
- Detector
- :UV (254 nm)
- Column temp.
- :40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Nonylphenol Ethoxylates (1) (GF-310 HQ)
- IGEPAL® CA-210 (Polyoxyethylene (2) Isooctylphenyl Ether) (KF-402HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (1) (GF-310 HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (2) (KF-402HQ)
- Brij® 52 (Polyoxyethylene(2) Cetyl Ether) (KF-402HQ)
- Brij® 56 (Polyoxyethylene(10) Cetyl Ether) (GF-310 HQ)
- Effects of Organic Solvent Concentration (1) (GF-310 HQ)
- Effects of Organic Solvent Concentration (2)
- Effects of Organic Solvents (GF-310 HQ)
- Degree of POE Polymerization (GF-310 HQ)
- Effects of Temperature (GF-310 HQ)
- Comparison with ODS Column (1)
- Comparison with ODS Column (2)
- Comparison with ODS Column (3)
- Triton X-100 (DS-413)
- Triton X-100 (SB-802.5 HQ)
- Triton X-100 (GF-310 HQ)
- Triton X-100 (GF-210 HQ)
- Polyoxethylene Sorbitan Monoleate (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (1) (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (2) (GF-310 HQ)
- Sodium Alkyl Sulfates (GF-310 HQ)
- Benzalkonium Chloride (1) (GF-310 HQ)
- Benzalkonium Chloride (2) (GF-310 HQ)
- Benzalkonium Chloride (3) (SB-802.5 HQ)
- Trimethylalkylammonium Chlorides (GF-310 HQ)
- Trimethylbenzylammonium Chloride (SB-802.5 HQ)
- Microchemical Analysis of Cationic Surfactants (GF-310 HQ)
- Simultaneous Analysis of Anionic and Non-ionic Surfactants
- Analysis of Various Tween Type Surfactants (GF-210 HQ)
- LC/MS Analysis of Linear Alkylbenzene Sulfonate (DE-213)
- LC/MS Analysis of Tween Type Surfactants in IgG (ODP2 HP-2D)
- LC/MS analysis of Polysorbate 20 in Antibody Drug Complex Preparation (ODP2 HP-2B)
- Analysis of Polysorbate 80 in Antibody Drugs (ODP2 HP-4D)
- Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-4D)
- LC/MS Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-2B)