Nnonylphenol ethoxylate, a nonionic surfactant was analyzed using Asahipak GF-310 HQ (an Aqueous/Organic SEC Column). The figure shows the effect of organic solvent concentartion on elution time for Nnonylphenol ethoxylate of different degree of POE polymerization. The elution characterstics of Nnonylphenol ethoxylate are different in the following three areas:
- Area 1:The organic solvent concentration is lower than 20 %. In this area, surfactants are adsorbed on the packing material and no peak appears in the chromatogram.
- Area 2:The organic solvent concentration is medium, 20 to 60 %. Surfactants are reteined by mainly hydrophobic interaction with packing maetrials and can be separated each other.
- Area 3:The organic solvent concentration is higher than 60 %. Surfactants cannot be retained by hydrophobic interaction with packing materials and elute as the single peak. This condition is good for microchemical analysis of trace of components.
Moreover, the elution characterstics of surfactants using GF-310 HQ has important features as follows:
- 1.The recoveries of surfactants are very high unless they are adsorbed. Refer to the table.
- 2.The surfactant of which molecular weight is larger elutes faster.
Sample: Nnonylphenol ethoxylate
Degree of POE polymerization (n) | Acetonitrile concentration in the eluent (%) | ||||
---|---|---|---|---|---|
0 | 25 | 50 | 75 | 100 | |
100 | Adsorption | 100 | 100 | 100 | 100 |
60 | Adsorption | 80 | 100 | 100 | 100 |
45 | Adsorption | Adsorption | 100 | 100 | 100 |
20 | Adsorption | Adsorption | 100 | 100 | 100 |
10 | Adsorption | Adsorption | 100 | 100 | 100 |
- Column
- :Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm)
- Flow rate
- :0.6 mL/min
- Detector
- :UV(280 nm)
- Column temp.
- :30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Nonylphenol Ethoxylates (1) (GF-310 HQ)
- IGEPAL® CA-210 (Polyoxyethylene (2) Isooctylphenyl Ether) (KF-402HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (1) (GF-310 HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (2) (KF-402HQ)
- Brij® 52 (Polyoxyethylene(2) Cetyl Ether) (KF-402HQ)
- Brij® 56 (Polyoxyethylene(10) Cetyl Ether) (GF-310 HQ)
- Effects of Organic Solvent Concentration (2)
- Effects of Organic Solvents (GF-310 HQ)
- Degree of POE Polymerization (GF-310 HQ)
- Effects of Temperature (GF-310 HQ)
- Comparison with ODS Column (1)
- Comparison with ODS Column (2)
- Comparison with ODS Column (3)
- Triton X-100 (DS-413)
- Triton X-100 (SB-802.5 HQ)
- Triton X-100 (GF-310 HQ)
- Triton X-100 (GF-210 HQ)
- Polyoxethylene Sorbitan Monoleate (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (1) (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (2) (GF-310 HQ)
- Sodium Alkyl Sulfates (GF-310 HQ)
- Benzalkonium Chloride (1) (GF-310 HQ)
- Benzalkonium Chloride (2) (GF-310 HQ)
- Benzalkonium Chloride (3) (SB-802.5 HQ)
- Trimethylalkylammonium Chlorides (GF-310 HQ)
- Trimethylbenzylammonium Chloride (SB-802.5 HQ)
- Microchemical Analysis of Cationic Surfactants (GF-310 HQ)
- Method Optimization for The Simultaneous Analysis of Anionic and Non-ionic Surfactants (GF-210 HQ)
- Simultaneous Analysis of Anionic and Non-ionic Surfactants
- Analysis of Various Tween Type Surfactants (GF-210 HQ)
- LC/MS Analysis of Linear Alkylbenzene Sulfonate (DE-213)
- LC/MS Analysis of Tween Type Surfactants in IgG (ODP2 HP-2D)
- LC/MS analysis of Polysorbate 20 in Antibody Drug Complex Preparation (ODP2 HP-2B)
- Analysis of Polysorbate 80 in Antibody Drugs (ODP2 HP-4D)
- Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-4D)
- LC/MS Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-2B)