Addition of approximately 50 mM of sodium nitrate or sodium chloride to an eluent and keeping in check effect of the electric charge of the sample make it possible to analyze anionic and cationic surfactants using Asahipak GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column).


Sample : 50 μL
Sodium dodecylbenzenesulfonate
(hard type) 100 mg/L

Column       : Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm)
Eluent       : (A); 50 mM NaNO3 aq./CH3CN=50/50
               (B); H2O/CH3CN=50/50
Flow rate    : 0.6 mL/min 
Detector     : UV (254 nm) 
Column temp. : 40 °C