Surfactants are categorized by their chemical structure into ionic and nonionic surfactants, but are characterized by the common feature that one molecule contains both hydrophilic and hydrophobic portions. Taking advantage of such a common feature, Asahipak GF-310 HQ, an Aqueous/Organic (multi-solvent use) SEC column, facilitates a wider range of separation for a variety of surfactants, which cannot be done by ODS columns. The separation can be made simply adjusting the concentration of the organic solvent or the salt in the eluent according to the purpose of the analysis. Moreover, since GF-310 HQ hardly swell or shrink in organic solvents, most of organic solvents can be used as the eluent and GF-310 HQ can be used for the analysis of surfactants of wide hydrophobicity range.