The comparison of Asahipak GF-310 HQ an Aqueous/Organic (multi-solvent use) SEC column) and an ODS column when they were used for the analysis of PEG (polyethylene glycol) is shown here.
When GF-310 HQ is used, the effect of acetonitrile concentration on elution time is rather small and the component of lager molecular weight elutes faster. Please refer to (A).
When an ODS column is used, the effect of acetonitrile concentration on elution time is very large and the component of smaller molecular weight elutes faster. The elution time can be shortened by increasing acetonitrile concentration. Please refer to (B).
When an ODS column is used for the analysis of surfactants, the effect of acetonitrile concentration on elution time is rather large and the component of lager molecular weight elutes faster. The elution time also can be shortened by increasing acetonitrile concentration. Please refer to (C).
From the results, we can say that the ODS column has hydrophobic interaction with both nonylphenyl groups and PEG and GF-310 HQ has hydrophobic interaction only with nonylphenyl groups. This difference is considered to be the cause of the difference in the measurement of POE distribution.
Sample :
(A),(B) PEG
(C) Nonylphenol ethoxylate
Column : (A) Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm) : (B),(C) ODS column from the other manufacturer Eluent : CH3CN/H2O Flow rate : (A); 0.6 mL/min (B),(C); 0.8 mL/min Detector : (A),(B); RI (C); UV (280 nm) Column temp. : 30 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Nonylphenol Ethoxylates (1) (GF-310 HQ)
- IGEPAL® CA-210 (Polyoxyethylene (2) Isooctylphenyl Ether) (KF-402HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (1) (GF-310 HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (2) (KF-402HQ)
- Brij® 52 (Polyoxyethylene(2) Cetyl Ether) (KF-402HQ)
- Brij® 56 (Polyoxyethylene(10) Cetyl Ether) (GF-310 HQ)
- Effects of Organic Solvent Concentration (1) (GF-310 HQ)
- Effects of Organic Solvent Concentration (2)
- Effects of Organic Solvents (GF-310 HQ)
- Degree of POE Polymerization (GF-310 HQ)
- Effects of Temperature (GF-310 HQ)
- Comparison with ODS Column (1)
- Comparison with ODS Column (2)
- Triton X-100 (DS-413)
- Triton X-100 (SB-802.5 HQ)
- Triton X-100 (GF-310 HQ)
- Triton X-100 (GF-210 HQ)
- Polyoxethylene Sorbitan Monoleate (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (1) (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (2) (GF-310 HQ)
- Sodium Alkyl Sulfates (GF-310 HQ)
- Benzalkonium Chloride (1) (GF-310 HQ)
- Benzalkonium Chloride (2) (GF-310 HQ)
- Benzalkonium Chloride (3) (SB-802.5 HQ)
- Trimethylalkylammonium Chlorides (GF-310 HQ)
- Trimethylbenzylammonium Chloride (SB-802.5 HQ)
- Microchemical Analysis of Cationic Surfactants (GF-310 HQ)
- Method Optimization for The Simultaneous Analysis of Anionic and Non-ionic Surfactants (GF-210 HQ)
- Simultaneous Analysis of Anionic and Non-ionic Surfactants
- Analysis of Various Tween Type Surfactants (GF-210 HQ)
- LC/MS Analysis of Linear Alkylbenzene Sulfonate (DE-213)
- LC/MS Analysis of Tween Type Surfactants in IgG (ODP2 HP-2D)
- LC/MS analysis of Polysorbate 20 in Antibody Drug Complex Preparation (ODP2 HP-2B)
- Analysis of Polysorbate 80 in Antibody Drugs (ODP2 HP-4D)
- Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-4D)
- LC/MS Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-2B)