Triton X-100 was analyzed using OHpak SB-802.5 HQ (a column for GFC separation). Triton X-100 is polyethyrene glycol mono-p-iso-octylphenyl ether and one of sufactants. It is used in the field of bio-chemistry. Its phydrophillic part is polyethylene glycol and the elution order is the order of chain length of poly(ethylene glycol) form 1 to 15,16 in the conditions of (B) and (C).



Sample : Triton X-100

Column       : Shodex OHpak SB-802.5 HQ (8.0 mm I.D. x 300 mm)
Eluent       : (A); H2O/CH3CN=30/70
               (B); H2O/CH3CN=70/30
               (C); H2O/CH3CN=80/20
Flow rate    : 0.5 mL/min
Detector     : UV (280 nm)
Column temp. : 60 °C