Triton X-100 (SB-802.5 HQ)

Triton X-100 was analyzed using OHpak SB-802.5 HQ (a column for GFC separation). Triton X-100 is polyethyrene glycol mono-p-iso-octylphenyl ether and one of sufactants. It is used in the field of bio-chemistry. Its phydrophillic part is polyethylene glycol and the elution order is the order of chain length of poly(ethylene glycol) form 1 to 15,16 in the conditions of (B) and (C).

Sample: Triton X-100

  1.  
Column
Shodex OHpak SB-802.5 HQ (8.0 mm I.D. x 300 mm)
Eluent
(A); H2O/CH3CN=30/70
(B); H2O/CH3CN=70/30
(C); H2O/CH3CN=80/20
Flow rate
0.5 mL/min
Detector
UV (280 nm)
Column temp.
60 ℃

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