Nonylphenol ethoxylater, a nonionic surfactant was analyzed using Asahipak GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column). The figure shows the chromatograms of different degree of POE polymerization. And, the table shows the elution time of the peaks.
Moreover, the elution characterstics of of alkylphenol ethoxylate and alkyl ethoxylate using GF-310 HQ has important features as follows:
- 1.The surfactant of which molecular weight is larger elutes faster.
- 2.The reproducibility of the elution time of the peaks are very good and are independent of the typical degree of POE polymerization of the sample.
These features are useful for the determination of the degree of POE polymerization.
Sample:
Nonylphenol ethoxylate
Typical degee of POE polymerization (n) | Degee of POE polymerization of each peak (n) | |||||
---|---|---|---|---|---|---|
10 | 6 | 4 | 3 | 2 | 1 | |
Monomer | - | - | - | 20.80 | 27.06 | 35.07 |
Dimer | - | 16.91 | 20.14 | 22.76 | 27.01 | 35.01 |
Hexamer | 13.99 | 16.96 | 20.09 | 22.70 | 22.01 | - |
Decamer | 14.15 | 16.91 | 19.99 | 22.70 | - | - |
- Column
- :Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm)
- Eluent
- :CH3CN/H2O=30/70
- Flow rate
- :0.6 mL/min
- Detector
- :UV(280 nm)
- Column temp.
- :30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Nonylphenol Ethoxylates (1) (GF-310 HQ)
- IGEPAL® CA-210 (Polyoxyethylene (2) Isooctylphenyl Ether) (KF-402HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (1) (GF-310 HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (2) (KF-402HQ)
- Brij® 52 (Polyoxyethylene(2) Cetyl Ether) (KF-402HQ)
- Brij® 56 (Polyoxyethylene(10) Cetyl Ether) (GF-310 HQ)
- Effects of Organic Solvent Concentration (1) (GF-310 HQ)
- Effects of Organic Solvent Concentration (2)
- Effects of Organic Solvents (GF-310 HQ)
- Effects of Temperature (GF-310 HQ)
- Comparison with ODS Column (1)
- Comparison with ODS Column (2)
- Comparison with ODS Column (3)
- Triton X-100 (DS-413)
- Triton X-100 (SB-802.5 HQ)
- Triton X-100 (GF-310 HQ)
- Triton X-100 (GF-210 HQ)
- Polyoxethylene Sorbitan Monoleate (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (1) (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (2) (GF-310 HQ)
- Sodium Alkyl Sulfates (GF-310 HQ)
- Benzalkonium Chloride (1) (GF-310 HQ)
- Benzalkonium Chloride (2) (GF-310 HQ)
- Benzalkonium Chloride (3) (SB-802.5 HQ)
- Trimethylalkylammonium Chlorides (GF-310 HQ)
- Trimethylbenzylammonium Chloride (SB-802.5 HQ)
- Microchemical Analysis of Cationic Surfactants (GF-310 HQ)
- Method Optimization for The Simultaneous Analysis of Anionic and Non-ionic Surfactants (GF-210 HQ)
- Simultaneous Analysis of Anionic and Non-ionic Surfactants
- Analysis of Various Tween Type Surfactants (GF-210 HQ)
- LC/MS Analysis of Linear Alkylbenzene Sulfonate (DE-213)
- LC/MS Analysis of Tween Type Surfactants in IgG (ODP2 HP-2D)
- LC/MS analysis of Polysorbate 20 in Antibody Drug Complex Preparation (ODP2 HP-2B)
- Analysis of Polysorbate 80 in Antibody Drugs (ODP2 HP-4D)
- Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-4D)
- LC/MS Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-2B)