Asahipak GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column) was used for an analysis of anionic surfactant. Anionic surfactants are often used in textile industries and as household detergents. The anion part of the compound works as a surfactant when ionized in a solvent. There are two types of sodium dodecylbenzensulfonate; linear type (soft type) and branched type (hard type). From an environmental pollution point of view, it is important to distinguish the two, since each type has different biodegradability and accumulation factors.
GF-310 HQ is feasible for distinguishing the soft and hard types without any complexity. There is no considerable difference in peak shapes of soft and hard types when analyzed at a mobile phase containing 60 % acetonitrile (Eluent I). However, analysis at lower acetonitrile concentration provides hydrophobic interaction affects and this creates differences in the peak shapes of soft and hard types, thus they can be distinguished (Eluent II and III).
Sample : 100 mg/L each, 20 μL
Sodium dodecylbenzenesulfonate (Soft type)
Sodium dodecylbenzenesulfonate (Hard type)
Column : Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm) Eluent : 50 mM NaCl aq./CH3CN Flow rate : 0.6 mL/min Detector : UV (220 nm) Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Nonylphenol Ethoxylates (1) (GF-310 HQ)
- IGEPAL® CA-210 (Polyoxyethylene (2) Isooctylphenyl Ether) (KF-402HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (1) (GF-310 HQ)
- Brij® 30 (Polyoxyethylene(4) Lauryl Ether) (2) (KF-402HQ)
- Brij® 52 (Polyoxyethylene(2) Cetyl Ether) (KF-402HQ)
- Brij® 56 (Polyoxyethylene(10) Cetyl Ether) (GF-310 HQ)
- Effects of Organic Solvent Concentration (1) (GF-310 HQ)
- Effects of Organic Solvent Concentration (2)
- Effects of Organic Solvents (GF-310 HQ)
- Degree of POE Polymerization (GF-310 HQ)
- Effects of Temperature (GF-310 HQ)
- Comparison with ODS Column (1)
- Comparison with ODS Column (2)
- Comparison with ODS Column (3)
- Triton X-100 (DS-413)
- Triton X-100 (SB-802.5 HQ)
- Triton X-100 (GF-310 HQ)
- Triton X-100 (GF-210 HQ)
- Polyoxethylene Sorbitan Monoleate (GF-310 HQ)
- Sodium Dodecylbenzenesulfonate (1) (GF-310 HQ)
- Sodium Alkyl Sulfates (GF-310 HQ)
- Benzalkonium Chloride (1) (GF-310 HQ)
- Benzalkonium Chloride (2) (GF-310 HQ)
- Benzalkonium Chloride (3) (SB-802.5 HQ)
- Trimethylalkylammonium Chlorides (GF-310 HQ)
- Trimethylbenzylammonium Chloride (SB-802.5 HQ)
- Microchemical Analysis of Cationic Surfactants (GF-310 HQ)
- Method Optimization for The Simultaneous Analysis of Anionic and Non-ionic Surfactants (GF-210 HQ)
- Simultaneous Analysis of Anionic and Non-ionic Surfactants
- Analysis of Various Tween Type Surfactants (GF-210 HQ)
- LC/MS Analysis of Linear Alkylbenzene Sulfonate (DE-213)
- LC/MS Analysis of Tween Type Surfactants in IgG (ODP2 HP-2D)
- LC/MS analysis of Polysorbate 20 in Antibody Drug Complex Preparation (ODP2 HP-2B)
- Analysis of Polysorbate 80 in Antibody Drugs (ODP2 HP-4D)
- Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-4D)
- LC/MS Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-2B)