Comparison with ODS Column (1)

The comparison of Asahipak GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column) and an ODS column when they were used for the measurement of POE distribution of octylphenol ethoxylate is shown here. When GF-310 HQ was used, good chromatograms were obtained using 35 and 40 % acetonitrile concentration. On the other hand, when an ODS column was used, POE distribution can be measured using 55 % acetonitrile concentration but the separation is not sufficient.

Sample :
Octylphenol ethoxylate

Column       : Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm)
Eluent       : CH3CN/H2O
Flow rate    : 0.6 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C
Column : ODS column from the other manufacturer Eluent : CH3CN/H2O Flow rate : 0.8 mL/min Detector : UV (280 nm) Column temp. : 30 °C

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