Oligopeptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).


Sample :
1. Val-Glu-Glu-Ala-Glu
2. Gly-Gly-Phe
3. Met-Enkephalin
4. Bradykinin
5. Angiotensin II
6. Angiotensin III
7. Phe-Phe-Phe

Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : (1): (A); 0.1 M NaClO4 + 0.1 % Sodium phosphate (pH2)
                    (B); CH3CN
               Linear gradient: 0 min to 60 min, (A)/(B)=90/10 to 60/40
               (2): (A); 0.1 M NaClO4 + 0.1 % Sodium phosphate (pH9)
                    (B); CH3CN
               Linear gradient: 0 min to 60 min, (A)/(B)=90/10 to 60/40
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : 30 °C