Oligopeptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography) with acidic and alkaline eluent.
Refer to Effects of Eluent pH (ODP-50 6D)
Sample:
- 1.Glu-Gly-Phe
- 2.Met-Enkephalin
- 3.Leu-Enkephalin
- 4.Leu-Trp-Met
- Column
- ：Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
- Eluent
- ：(1); 0.05 % TFA aq./CH3CN=85/15
(2); 50 mM Ammonium acetate buffer(pH10.0)/CH3CN=85/15 - Flow rate
- ：1.0 mL/min
- Detector
- ：UV (220 nm)
- Column temp.
- ：30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Standard Proteins (4) (C4P-50 4D)
- Comparison of Silica-based Column with Polymer-based Column
- Proteins and Peptides (ODP-50 6D)
- Recovery of Peptides (ODP-50 6D)
- Basic Peptides
- Effects of Eluent pH (ODP-50 6D)
- Oligopeptides (1)
- Oligopeptides (2)
- Dipeptides (DE-613)
- Angiotensins
- Opioid Peptides (1) (By Post-column Reaction Method)
- Opioid Peptides (2) (By Pre-column Reaction Method)
- Tryptic Digest of Myoglobin (2) (ODP-50)
- Analysis of Glutathiones (By Post-column Reaction Method)
- Analysis of Lactoferrin Supplement (C4P-50 4D)
- Analysis of Lactoferrin Concentrate According to Analytical Methods of Food Additives in Foods (C4P-50 4D)