Three kinds of protein standards were analyzed using Asahipak C4P-50 4D (a column for reversed phase chromatography).


Sample :
1. BSA
2. Chymotrypsinogen A
3. Ferritin

Column       : Shodex Asahipak C4P-50 4D (4.6 mm I.D. x 150 mm)
Eluent       : (A); 0.1 % TFA aq./CH3CN=90/10
               (B); 0.1 % TFA aq./CH3CN=5/95
                Linear gradient: 0 to 30 min, (A) to (B)
Flow rate    : 0.6 mL/min
Detector     : UV (280 nm)
Column temp. : 30 °C