Reduced glutathione (GSH) and oxidized glutathione (GSSG) are separated with using polymer based reversed phase column, Asahipak ODP-50 6E .


Sample : 5 ppm each,
1. Glutathione , GSH (MW: 307.33)
2. Glutathione disulfide, GSSG (MW: 612.63)

Column       : Shodex Asahipak ODP-50 6E (6.0 mm I.D. x 250 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH2.5)
Reagent      : 50 mM o-Phthalaldehyde in CH3OH/200 mM Sodium phoshate buffer (pH12.0)/
               2-Mercaptoethanol=50/450/1
Flow rate    : (eluent); 0.5 mL/min, (reagent); 0.3 mL/min
Detector     : Fluorescnce (Ex. 340 nm, Em. 420 nm)
Column temp. : 40 °C