Lactoferrin is a protein found in many mammalian milks, especially in the first breast milks. According to the Quantitative Method for “Lactoferrin Concentrate” listed in the 9th Edition of the Japanese Specifications and Standards for Food Additives (JSFA-IX*), the method should be carried out with a column filled with butylated polyvinyl alcohol polymer gel. In this application, lactoferrin standard and lactoferrin concentrate were analyzed using Asahipak C4P-50 4D, a polymer-based column for reversed phase chromatography. The JSFA states that lactoferrin concentrates to contain 85% or more of lactoferrin. It was confirmed that the sample analyzed met this requirement.

Sample Preparation

1. (1)Weight 0.1 g of commercial lactoferrin concentrates.
2. (2)Dissolve in 0.3 % NaCl aqueous solution to make exactly 50 mL, and use it as the sample solution.

*The version at the time of the application acquisition.

Sample: 25 µL
(Standard solution) 2 mg/mL of Lactoferrin in 0.3 % NaCl aq.
(Sample solution) Lactoferrin Concentrates in 0.3 % NaCl aq.

1. 1.Lactoferrin

 

1.