Recovery of Peptides (ODP-50 6D)

In analyzing peptides using reversed phase columns (particularly ODS columns), there is always a concern about a decline in recovery. Therefore Asahipak ODP series should be used, which shows a better recovery in analyzing peptides of high, medium and low molecular weights as well as in analyzing acidic, neutral or basic peptides. The recovery of a number of peptides with a wide range of different characteristics are shown for isocratic elution conditions. With these columns it is possible to get a good recovery by isocratic elution.

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Substances (1 μg each) MW pI HP Recovery (%)
Eluent A Eluent C Eluent C
Glu-Gly-Phe peptideic peptide   351   3.29   2.17   96 103   88
Substance P Frg.1-4 Basic peptide   497 11.44   3.24 100 100 110
Met-Enkephalin Neutral peptide   574   5.90   5.02   96   96   99
Des-Arg9-Bradykinin Basic peptide   904 11.05   7.65 -   98 102
Substance P Basic peptide 1348 12.00   8.61 -   90 105
α-Endorphin Neutral peptide 1684   6.85   6.94 -   96 100
β-Endorphin Basic peptide 3465   9.93 16.80 - - 100
Insulin B chain S-sulphonate Neutral peptide 3496   6.05 24.05 - -   97
Big gastrin (Human) peptideic peptide 3749   4.32 23.53 - -   95
PI: Isocratic point
HP: Hydrophorbic Parameter
Column
：Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent
：(A); 0.05 % TFA aq./CH3CN=85/15
(B); 0.05 % TFA aq./CH3CN=80/20
(C); 0.05 % TFA aq./CH3CN=70/30
Flow rate
：1.0 mL/min
Detector
：UV (220 nm)
Column temp.
：30 ℃

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