Opioid peptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography) by post-column reaction method.
Sample :
1. Tyr-Gly
2. Tyr-Gly-Gly
3. Tyr-Gly-Gly-Phe
4. Tyr-Phe
5. ME-Thr-ser-Glu-Lys
6. ME, Met-Enkephalin
7. LE, Leu-Enkephalin
8. ME-Arg, Met-Enkephalin-Arg
9. α-EDP, α-Endorphin
10. LE-Arg, Leu-Enkephalin-Arg
11. ME-NH2, Met-Enkephalin-NH2
12. γ-EDP, α-EDP-Leu
13. ME-Arg-Glu-Leu
14. LE-NH2, Leu-Enkephalin-NH2
15. ME-Arg-Phe
16. DNP 1-8
Column : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm) Eluent : (A); 50 mM Sodium borate (pH10.0)/H2O/CH3CN=4/15/1 (B); 50 mM Sodium borate (pH10.0)/H2O/CH3CN=1/1/3 Gradient: (A) to (B) Flow rate : 1.0 mL/min Detector : Fluorescnce (Ex. 330 nm, Em. 430 nm) Column temp. : Room temp.
Guo-Qing Zhang et al., Analytical Science, 6 (1990) 671.
Sample Name Index
- Endorphin
- Leu-Enkephalin
- Leu-Enkephalin-Arg
- Leu-Enkephalin-NH2
- Met-Enkephalin
- Met-Enkephalin-Arg
- Met-Enkephalin-Arg-Gly-Leu
- Met-Enkephalin-Arg-Phe
- Met-Enkephalin-NH2
- Met-Enkephalin-Thr-Ser-Glu-Lys
- Tyr-Gly
- Tyr-Gly-Gly
- Tyr-Gly-Gly-Phe
- Tyr-Phe
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Standard Proteins (4) (C4P-50 4D)
- Comparison of Silica-based Column with Polymer-based Column
- Proteins and Peptides (ODP-50 6D)
- Recovery of Peptides (ODP-50 6D)
- Basic Peptides
- Effects of Eluent pH (ODP-50 6D)
- Analysis of Oligopeptides with Acidic and Alkaline Eluent (ODP-50 6D)
- Oligopeptides (1)
- Oligopeptides (2)
- Dipeptides (DE-613)
- Angiotensins
- Opioid Peptides (2) (By Pre-column Reaction Method)
- Tryptic Digest of Myoglobin (2) (ODP-50)
- Analysis of Glutathiones (By Post-column Reaction Method)
- Analysis of Lactoferrin Supplement (C4P-50 4D)
- Analysis of Lactoferrin Concentrate According to Analytical Methods of Food Additives in Foods (C4P-50 4D)