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Proteins and Peptides

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Using gradient elution, a sample containing proteins and peptides with a variety of molecular weight were separated. Asahipak ODP series provides good reproducibility of elution volume as well as high recovery for analysis of proteins and peptides.

No.Proteins
Peptides
MWVR(mL)
First
Analysis
VR(mL)
Second
Analysis
Recovery (%)
1Lys-Bradykinin 1188 4.63 4.64 97
2Bradykinin 1060 5.73 5.70 92
3Met-Enkephalin 574 7.00 7.00 97
4Neurotensin 1673 8.04 8.05 99
5Leu-Enkephalin 556 8.47 8.48 100
6Substance P 1348 9.24 9.26 93
7Bacitracin 1450 11.47 11.48 81
8Insulin 5750 12.24 12.25 95
9Insulin B chain 3476 12.87 12.89 91
10Lysozyme 14300 14.43 14.44 96
11Mastoparan 1479 15.97 15.98 96
12Myoglobin 17500 18.17 18.18 83

Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : 0.05 % TFA aq./CH3CN
               Linear gradient: 80/20 to 50/50, 20 min
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : 30 °C

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