Proteins and Peptides (ODP-50 6D)

Using gradient elution, a sample containing proteins and peptides with a variety of molecular weight were separated. Asahipak ODP-50 6D provides good reproducibility of elution volume as well as high recovery for analysis of proteins and peptides.

 

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No. Proteins/Peptides MW Elution volume (mL) Recovery (%)
1st Analysis 2nd Analysis
1 Lys-Bradykinin   1188   4.63   4.64   97
2 Bradykinin   1060   5.73   5.70   92
3 Met-Enkephalin     574   7.00   7.00   97
4 Neurotensin   1673   8.04   8.05   99
5 Leu-Enkephalin     556   8.47   8.48 100
6 Substance P  1348   9.24   9.26   93
7 Bacitracin   1450 11.47 11.48   81
8 Insulin   5750 12.24 12.25   95
9 Insulin B chain   3476 12.87 12.89   91
10 Lysozyme 14300 14.43 14.44   96
11 Mastoparan   1479 15.97 15.98   96
12 Myoglobin 17500 18.17 18.18   83
Column
：Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent
：0.05 % TFA aq./CH3CN
Linear gradient; 80/20 to 50/50, 20 min
Flow rate
：1.0 mL/min
Detector
：UV (220 nm)
Column temp.
：30 ℃

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Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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