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Tryptic Digest of Myoglobin (2) (ODP-50)

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Tryptic digest of myoglobin were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).


Sample : Tryptic digest of Myoglobin


Abbreviation of Amino Acids

Abbreviation of Amino Acid
A Ala M Met
C Cys N Asn
D Asp P Pro
E Glu Q Gln
F Phe R Arg
G Gly S Ser
H His T Thr
I Ile V Val
K Lys W Trp
L Leu Y Tyr
Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : (A); 0.07 % TFA aq.
               (B); CH3CN containing 0.05 % TFA
               Initial ratio (A)/(B)=90/10
               Linear gradient of 1 %/min
Flow rate    : 0.8 mL/min
Detector     : UV (214 nm)
Column temp. : 30 °C

Coutesy of Dr. Takano, Institute for Protein Research, Osaka University

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