Tryptic digest of myoglobin were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography).

Sample : Tryptic digest of Myoglobin
(Amino acid sequence of Myoglobin)

| Abbreviation of Amino Acid | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| A | Ala | C | Cys | D | Asp | E | Glu | F | Phe |
| G | Gly | H | His | I | Ile | K | Lys | L | Leu |
| M | Met | N | Asn | P | Pro | Q | Gln | R | Arg |
| S | Ser | T | Thr | V | Val | W | Trp | Y | Tyr |
Column : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent : (A); 0.07 % TFA aq.
(B); CH3CN containing 0.05 % TFA
Initial ratio (A)/(B)=90/10
Linear gradient of 1 %/min
Flow rate : 0.8 mL/min
Detector : UV (214 nm)
Column temp. : 30 °C
Coutesy of Dr. Takano, Institute for Protein Research, Osaka University
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Standard Proteins (4) (C4P-50 4D)
- Comparison of Silica-based Column with Polymer-based Column
- Proteins and Peptides (ODP-50 6D)
- Recovery of Peptides (ODP-50 6D)
- Basic Peptides
- Effects of Eluent pH (ODP-50 6D)
- Analysis of Oligopeptides with Acidic and Alkaline Eluent (ODP-50 6D)
- Oligopeptides (1)
- Oligopeptides (2)
- Dipeptides (DE-613)
- Angiotensins
- Opioid Peptides (1) (By Post-column Reaction Method)
- Opioid Peptides (2) (By Pre-column Reaction Method)
- Analysis of Glutathiones (By Post-column Reaction Method)
- Analysis of Lactoferrin Supplement (C4P-50 4D)
- Analysis of Lactoferrin Concentrates According to JSFA Method (C4P-50 4D)
